WP5 - Identification of the Molecular Mechanisms of Action

Objectives

  • To evaluate the neuroprotective effects of brimonidine and SST on retinal neurons cultured under conditions that mimic the deleterious environment of diabetic metabolism: high glucose and glutamate-induced excitotoxicity, with and without the added component of hypoxia.
  • To define the effects of brimonidine and SST on mitochondrial biogenesis (the balance between mitochondrial biogenesis and mitochondrial fission) under conditions of oxidative stress.
  • To complement the clinical observations of briminodine/SST therapy with mechanistic data derived from in vitro studies.